High-Throughput and High-Dimensional Single Cell Analysis of Antigen-Specific CD8+ T Cells

We developed a high-dimensional, tetramer associated T cell receptor sequencing (TetTCR-SeqHD) method to simultaneously profile cognate antigen specificities, TCR sequences, targeted gene-expression, and surface protein expression. Cultured antigen-specific CD8+ T cell clones were studied as a proof-of-concept experiment. In addition, both the antigen specific CD8+ T cells from healthy subjects and Type I diabetic patients were investigated. 18 individually consented subjects pooled into four groups for sample processing]]> PBMCs from healthy subjects and Type I diabetic patients were included in the study. ]]>