Targeted bisulfite sequencing

Raw reads from targeted bisulfite sequencing. The SureSelect Methyl-Seq target enrichment system (Agilent Technologies, Inc., CA, USA) was used to prepare bisulfite sequencing libraries from blood DNA samples of lymphoma patients (Ly1, Ly2), healthy family members (Ly8, Ly9, Ly10, Ly11, Ly12, Ly13 and Ly14), baseline controls (Control1-5), DNMT3A mutation carriers (Id5, Id7, Id9, Id11) and their age-matched controls (Id6, Id8, Id10, Id12). In addition, blood DNA sample of a patient (HLRCC_N7) with germline fumarate hydratase (FH) mutation is included. Illumina paired-end sequencing for targeted libraries from Ly1, Ly2, Ly8, Ly9, Ly10 and Ly11 was done at Karolinska Institutet using 100 base-pair read length and the HiSeq2000 platform. Illumina paired-end sequencing for targeted libraries from Ly12, Ly13, Ly14, and DNMT3A mutation carriers and their age-matched controls was done as a service at BGI (BGI Tech Solutions Co., Ltd., China) using 126 base-pair read length and the HiSeq2500 platform.

本数据集包含针对特定区域亚硫酸氢盐测序的原始测序读数。采用Agilent Technologies, Inc., CA, USA公司生产的SureSelect Methyl-Seq靶向富集系统，从淋巴瘤患者（Ly1, Ly2）、健康家庭成员（Ly8, Ly9, Ly10, Ly11, Ly12, Ly13和Ly14）、基线对照者（Control1-5）、DNMT3A突变携带者（Id5, Id7, Id9, Id11）及其年龄匹配对照者（Id6, Id8, Id10, Id12）的血液DNA样本中制备亚硫酸氢盐测序文库。此外，还包括了一位具有胚系富马酸水合酶（FH）突变的患者（HLRCC_N7）的血液DNA样本。针对Ly1, Ly2, Ly8, Ly9, Ly10和Ly11的靶向文库，在Karolinska Institutet进行了Illumina双端测序，测序长度为100碱基，使用HiSeq2000平台。针对Ly12, Ly13, Ly14、DNMT3A突变携带者及其年龄匹配对照者的靶向文库，则在BGI（BGI Tech Solutions Co., Ltd., China）以服务形式进行Illumina双端测序，测序长度为126碱基，使用HiSeq2500平台。