AMR Gene identification in Proteus mirabilis, Escherichia coli, Staphylococcus saprophyticus, Staphylococcus arlettae and Enterococcus avium Isolates from Poultry fecal, Environmental, and Human fecal Samples

Antimicrobial resistance (AMR) is a growing global health challenge, especially at the human-animal-environment interface, where resistant bacteria may be transferred along food chains and through ecosystems. This study undertakes genomic characterization of Escherichia coli, Enterococcus and Staphylococcus isolated from poultry, human, and environmental hosts with the aim of identifying their resistance mechanisms. Whole Genome Sequencing (WGS) of E.coli, Staphylococcus arlettae, Proteus Mirabilis, Enterococcus avium and Staphylococcus saprophyticus carried out for the identification of AMR genes, virulence factors, and possible horizontal gene transfer (HT) events. Comparative analysis of WGS data with antibiotic susceptibility testing (AST) provided insight into genotype-phenotype correlations and resistance patterns. The results emphasize developed robust AMR surveillance systems and effective intervention strategies. Genome sequences would be made available publicly, whereby contributing to global efforts in AMR research and monitoring.