Spatially clustered type I interferon responses at injury borderzones [scRNA-Seq]

Sterile inflammation post-myocardial infarction was thought to be driven by myeloid cells, however, we show that borderzone cardiomyocytes are the critical initators of the Type I Interferon response through spatial, single-cell, and single-nuclei transcriptomics. Nuclei and cells were isolated from 12-14-week-old adult wild-type C57BL/6J, IFNAR-/-, and IRF3-/- mice. For whole cell suspensions, freshly harvested hearts were enzymatically digested prior to filtering and flow cytometry sorting. Nuclei were isolated from fressh frozen tissue, minced and lysed, then filtered priored to collection.