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Calcium waves in MDCK epithelium

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https://www.omicsdi.org/dataset/bioimages/S-BIAD1135
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This dataset shows calcium signalling waves in a MDCK epithelium. The cells stably express GCaMP6S, a GFP-based intracellular calcium sensor. Imaging data courtesy of Yasuyuki Fujita (Y. Takeuchi et al., “Calcium Wave Promotes Cell Extrusion,” Current Biology, vol. 30, no. 4. Elsevier BV, pp. 670-681.e6, Feb. 2020. doi: 10.1016/j.cub.2019.11.089). Collective event analysis has been performed using ARCOS (P. A. Gagliardi et al., “Automatic detection of spatio-temporal signaling patterns in cell collectives,” Journal of Cell Biology, vol. 222, no. 10. Rockefeller University Press, Jul. 27, 2023. doi: 10.1083/jcb.202207048). The analysis scripts and results corresponding to the raw data published here are accessible online (Maciej Dobrzynski, “ARCOS, a computational approach for automatic recognition of collective signalling.” Mendeley, Apr. 26, 2023. doi: 10.17632/8GCNCG6ZKT.3). Additionally, the dataset has been used to showcase the capabilities of conv-paint, an interactive pixel classification tool for napari. Data dimensions: [t,y,x] -> [300,1024,1024] Pixel resolution: 0.86µm/px (880.64μm total) Time resolution: 5sec/frame (25min total) Filetype: TIFF
创建时间:
2024-08-23
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