RNAseq on Drosophila larvae genetically adapted to poor diet in microbiota-colonized and germ-free state

The study uses experimental evolution; it aims at understanding evolutionary adaptation to chronic nutritional stress and the role of microbiota in this process. Six outbred populations of Drosophila melanogaster (SEL1-SEL6) have been maintained on a poor larval diet for >190 generations, six control populations (CTL1-CTL6) were maintained in parallel on standard diet. We performed RNAseq on late 3rd instar larvae from both sets of populations under germ-free condition and when they were experimentally colonized by microbiota. The detailed methods and results are reported in Erkosar, B., S. Kolly, J. R. van der Meer, and T. J. Kawecki. 2017. Adaptation to chronic nutritional stress leads to reduced dependence on microbiota in Drosophila. mBio 8:e01496-01417, doi:10.1128/mBio.01496-17