Fig3F_STOML2_ExtFig5F_BCAP31_RACE_R2_LG302.sgd

HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with a 99 bp inserted sequences from the STOML2 either wild-type or containing a GCTG --> TAGC mutation. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with a 99 bp inserted sequences from the STOML2 or BCAP31 genes, either wild-type or containing a GCTG --> TAGC mutation. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.