Drug synergy slows ageing and improves healthspan through IGF and SREBP lipid signaling.. Caenorhabditis elegans strain:N2 and eat-2

After two days of treatment with drug or vehicle RNA was extracted. For library preparation an amount of 2μg of total RNA per sample was processed using Illumina’s RNA Sample Prep Kit following the manufacturer’s instruction (Illumina, San Diego, CA, USA). All single drugs were processed together and multiplexed onto one lane to minimize batch effect. The different drug combinations were processed into two multiplexed lanes. In total, we ran three lanes in parallel with 20 samples in each and we had untreated N2 controls in each lane. Libraries were sequenced using Illumina HiSeq4000 sequencing platform (Illumina, San Diego, CA, USA) in a paired end read approach at a read length of 150 nucleotides. Sequence data was extracted in FastQ format.