Gene expression analysis of mouse ID8 ovarian cancer cells stimulated with CCL6 in vitro

We used mouse Clariom-S microarrays to study the gene expression profile of ID8 cancer cells stimulated with C-C Chemokine Ligand 6 (CCL6). RNA extracted from ID8 mouse ovarian cancer cell lines (n=3) and ID8 cells stimulated with 200ng/mL CCL6 for 6 hours (n=3) was hybridized on Affymetrix Mouse Clariom S microarrays. We sought to identify key regulatory pathways activated in ovarian cancer cells upon stimulation with CCL6 that play a role in migration and metastasis. To understand the signaling mechanism of CCR1-CCL6 interaction in ovarian cancer, we generated biological replicates for unstimulated ID8 cells and ID8 cells stimulated with 200ng/mL CCL6 for 6 hours.