The goal of the study was to determine the role of a PLA2 enzyme called Pat1 using a Rickettsia parkeri strain with a transposon insertion in the pat1 gene.
#Titer below the limit of detection was denoted as one-half the detection limitNA: Revertant frequency not calculated because strains showed no Su sensitivity.
Five λKAN lysogenic colonies formed by the twelve resistant mutants were tested for their susceptibility to λVIR, as evaluated by a spot test assay. The rows shaded in green correspond to strains that
Errors indicated between brackets are the standard error of the mean of 3 independent experiments. No statistically significant differences (p-value <0.05) have been found between strains from the sam
1Isogenic E. coli hosts (RF146 or RF335) harboring either wild type SXT or most SXT were used in these assays.2Calculated using QPCR by measuring the ratio of attB: 3′prfC (see [20]).3Calculated as th