Generation of Th1/Th17 mediated immunity in response to <i>C.</i><i>difficile</i> in an <i>in-vitro</i> murine model system.

WT BMDCs were infected with PFA-fixed C. difficile strains. 24 h post-infection, stimulated BMDCs were co-cultured with WT splenocytes in the presence of anti-CD3/CD28. 96 h post co-culture, secreted cytokines IFN-γ (A), IL-17A (B), IL-10 (C), IL-4 (D) and IL-2 (E) were measured by ELISA. 1 µg/ml LPS stimulation served as reference control. Data represent mean ± SEM, n = 3. */∧p<0.05, **/∧∧p<0.01 and ***p<0.001 represent significant difference when compared to uninfected control cells and significant inter-strain difference, respectively. P values were obtained using ANOVA with Bonferroni post-test analysis.