Dual benefit for the gut and liver: Bifidobacterium breve BBr60 improves gut microbiota balance and hepatic glucose metabolism in a high-fat diet-induced obese mouse model

The aim of this study was to investigate the regulatory effect of Bifidobacterium breve BBr60 on gut microbiota dysbiosis and associated metabolic abnormalities in an obese mouse model induced by a high-fat diet (HFD) and to evaluate its potential impact on the balance of the gut microbiome.Mice were divided into a normal diet (CTL) group, a high-fat diet (HFD) group, and a BBr60 intervention group for an 8-week experiment. During this period, we monitored the mice's weight, lipid levels, markers of glucose metabolism and gut microbiota composition. Oil red O staining, hematoxylin and eosin staining, RT-PCR analysis, and high-throughput sequencing techniques were used to examine liver lesions, inflammation levels, tight junction protein expression, and gut microbiota diversity and richness.BBr60 significantly ameliorated weight gain, glucose metabolism abnormalities, lipid metabolism abnormalities, and liver tissue lesions in mice induced by HFD. In addition, BBr60 effectively attenuated HFD-induced inflammation and increased intestinal permeability. In terms of gut microbiota composition, BBr60 reversed HFD-induced dysbiosis, particularly by decreasing the relative abundance of opportunistic pathogens such as Duncaniella and increasing beneficial bacteria such as Clostridium_XIVa, Limosilactobacillus and Bifidobacterium. Time-dynamic analysis showed that BBr60 can regulate the relative abundance of the gut microbiota and improve HFD-induced dysbiosis of the gut microbiota.BBr60 significantly ameliorated metabolic abnormalities and gut microbiota imbalance in an obese mouse model induced by HFD. These results suggest that BBr60 has the potential to serve as an intervention for obesity-related metabolic abnormalities and gut microbiota imbalance. Further research may reveal the potential of BBr60 for clinical applications, particularly for the treatment of obesity and its associated complications.