Additional file 3 of Benefiting from the past: establishing in vitro culture of European beech (Fagus sylvatica L.) from provenance trial trees and seedlings

Additional file 3. Evaluation of beech seedlings 12 weeks after in vitro culture establishment. Five different media (M0, M2, M2.1, M2.2, M3) were tested using whole shoots (WS) or shoot tips (ST) of seedlings. The WS and ST or media were either separately evaluated or combined (pooled). The percentages of explants exhibiting shoot formation, bacterial or fungal contamination, resting bud formation, and chlorosis are given. The median was calculated for shoot length and the number of shoots derived from one explant. Values labelled with different letters indicate significant differences between media/explant types according to pairwise Dunn’s test for the number of shoots or shoot length and Pearson’s chi‒squared test for bacterial contamination, fungal contamination, resting bud formation, and chlorosis with p ≤ 0.05. IQR – interquartile range.