Marine vesicles: one more layer of complexity in the dissolved organic fraction

These data include the genome of the Vibrio kanaloae strain, which was isolated in pure culture from a seawater sample collected on May 31, 2022, from Cape Huertas (Alicante, Spain). The genome was sequenced using PacBio HiFi long reads. Additionally,  reads sequences from the extracellular vesicles (EVs) in the 20% OptiPrep fraction were isolated from the Vibrio kanaloae culture incubated in dialysis tubing cellulose membranes (Sigma-Aldrich, ref: D9652-100FT, width: 33 mm, 14 kDa). These membranes were immersed in an aquarium filled with natural seawater collected on December 9, 2022, from Cape Huertas (Mediterranean Sea, Alicante) and incubated at room temperature (approximately 18–20°C) for 1 week.

EVs_20_rep1_AQ_1C_culture_1 represents reads of the EVs 20% fraction isolated from the Vibrio kanaloae strain 1C aquarium culture, with rep1 and rep2 denoting the first and second replicates, respectively.

The EV reads were analyzed to facilitate a comprehensive evaluation of the representation and distribution of the mapped reads across the Vibrio genome.

This mapping approach enables the discernment of whether the mapped reads exhibit a uniform distribution, characteristic of a random DNA release process, or a non-uniform distribution, indicating a proclivity for alignment with specific genetic loci within the Vibrio genome. This distinction is crucial for unraveling the underlying intricacies governing DNA release and transfer in Vibrio kanaloae, thereby advancing our understanding of these complex processes.