Basal levels of CYP1 and AHR1 mRNA expression in chicken embryos on developmental day 7.

Samples of whole-body, yolk sac membrane (YSM) and chorioallantoic membrane (CAM) were collected from unexposed 7-day-old chicken embryos and analyzed by real-time RT-PCR (n = 3). Results are shown as non-normalized data calculated by E−Ct×106. Each sample contained cDNA prepared from 30 ng of total RNA. The highest values among the CYP1s in a sample type are shown in bold.