Analysis of anti-CD3e-induced transcriptional changes in WNK1-deficient thymocytes

RNAseq was used to analyse transcriptional changes occuring in WNK1-expressing or WNK1-deficient DN3 thymocytes following injection of anti-CD3e Overall design: Wnk1fl/+;CD2Cre;Rag1KO (HET, WNK1-expressing) or Wnk1fl/fl;CD2Cre;Rag1KO (KO, WNK1-deficient) mice were injected intraperitoneally with anti-CD3e and thymi was harvested after 3, 24 and 48 hours. Uninjected mice were used for the 0 hour timepoint. Thy1.2+, CD44- thymocytes were sorted by flow cytometry and RNA was extracted and sequenced. There were three replicates for each genotype/time point except for 4 replicates for WNK1-deficient mice at 0 h time point.