Total RNA Sequencing of control and huntington's disease iPSC-derived medium spiny neuron-like cells

HD and control patient-derived induced pluripotent stem cells were used to generate medium spiny neuron (MSN)-like cells. Three control in triplicate, one control in duplicate, one HD samples with CAG repeat length in typical adult onset range in triplicate and one in duplicate, and five HD samples with CAG repeat length in typical juvenile onset range in triplicate were differentiated as biological growth replicate (separate differentiations) into medium spiny neuron-like cells. Total RNA was isolated using the Qiagen RNeasy Kit and QIAshredders for cell lysis. 1 µg of RNA with RIN values >9 were used for library preparation using the strand specific Illumina TruSeq Total RNA protocol. Libraries were sequenced on the HiSeq 2500 using 100 cycles to obtain paired-end 100 reads at >50M reads per sample. Overall design: Total RNAseq profiles of control and HD patient iPSC-derived medium spiny neuron-like cells were generated by deep sequencing, in duplicate or triplicate, using Illumina Hiseq 2500 Rep 1/2/3/4 are all different human subjects except for the 18n2 and 18n6 samples which are the same person but 2 different iPSC clones. Thus, each Rep represents a different iPS cell line. Appended to the end of the title are _1/_2/_3 which represent different differentiations of those subjects' iPSCs and are separate RNA extractions. The rep number and appended differentation number are available in the 'source name' field on each sample page.