An effective protocol for analyzing the Anxiolytic Effects of bioactive oligosaccharide and Reverse the Impact of Chronic physical Stress in Mice based on Gut microbiota modulation

·
In vivo evaluation - Antistress efficacy in mice Model
Animals
and maintenance protocol
Physical
stress induction in mice
Weight
and feed intake measurement
·
Behaviour analysis based on stress induction
Elevated
plus-maze test
Open-field
test
Novel
object recognition test
·
Neurotransmitter - Corticosterone and serotonin Levels determination
·
Screening of microbiota modulation based on metagenomics analysis
Next-generation
sequence (NGS) - Illumina sequence workflow
NGS data analysis
Quality assessment
and sample sequencing
Metagenomics Data
Analysis

Data
filtering and Normalization