An effective protocol for analyzing the Anxiolytic Effects of bioactive oligosaccharide and Reverse the Impact of Chronic physical Stress in Mice based on Gut microbiota modulation

· In vivo evaluation - Antistress efficacy in mice Model Animals and maintenance protocol Physical stress induction in mice Weight and feed intake measurement · Behaviour analysis based on stress induction Elevated plus-maze test Open-field test Novel object recognition test · Neurotransmitter - Corticosterone and serotonin Levels determination · Screening of microbiota modulation based on metagenomics analysis Next-generation sequence (NGS) - Illumina sequence workflow NGS data analysis Quality assessment and sample sequencing Metagenomics Data Analysis Data filtering and Normalization