A study of A bacterial host factor confines phage localization for excluding the infected compartment through cell division. Pollak Fiyaksel et al.

This dataset supports the research described in our manuscript:
"A bacterial host factor confines phage localization for excluding the infected compartment through cell division."

Our hypothesis was that Bacillus subtilis actively restricts bacteriophage infection through a host-driven mechanism. The data demonstrates that the bacterial protein YjbH binds to the injected phage DNA and recruits the cell division machinery to isolate the infected region via asymmetric division, ultimately confining the spread of viral components.

The dataset includes:

* Raw and processed images from fluorescence and confocal microscopy, tracking phage infection dynamics.
* Quantifications of plaque size, lysis curves, and protein–DNA binding assays (e.g., EMSA, ddPCR).
* Western blot data for protein expression validation.
* Cryo-ET tomograms showing asymmetric septa formation.
* DNA-protein interaction data, including co-IP and MS results.

Each dataset is labeled by figure and panel, and includes a short description to facilitate interpretation.