Effects of colchicine on cardiac pyroptosis following CoCl2 exposure

This experiment was conducted using the H9c2 cardiac myoblast cell line. This study compared the H9c2 cell line under hypoxic conditions to those treated with both hypoxia and colchicine.Sheet 1: HIF-1 alpha-positive cells (flowcytometry analysis) on various concentrations of cobalt chloride and time pointsSheet 2: Cell viability (WST-1 assay) on various concentrations of cobalt chloride and time pointsSheet 3: Expression of HIF-1 alpha, IL-18, caspase-1, and NFkB following early (3 h) and late (24 h) hypoxia) using flowcytometry analysis. Cobalt chloride exposure was 600 micro Molar and the colchicine treatment was 1 micro Molar.Sheet 4: The percentage of pyroptotic H9c2 cells analyzed by flowcytometry after 3 h and 24 h hypoxic treatment. Early pyroptosis indicated cells expressing only caspase-1, while late pyroptosis indicated cells expressing both caspase-1 and propidium iodide.Sheet 5: Similar analysis as sheet 4, but the experiment was performed using confocal laser scanning microscope. Data are presented as arbitrary unit.