In vitro growth inhibition by pazopanib on breast carcinoma and melanoma cell lines expressing different genotypes in the Ras-Raf-ERK1/2 pathway*.

*Genotypes for MDA-MB-231 and MCF7 [47]; genotypes for WM3899 and WM3918 personal communication by Dr. Meenhard Herlyn; genotypes for SKMEL2 and SKMEL28 [48].†IC50 measured using a trypan blue exclusion cell viability assay 96 h after pazopanib treatment.∥Determined by western blot analysis. (+) when a clear band appeared in less than one minute of exposure; (−) when no signal at all appeared after more than 30 min of exposure; (+/−) when a faint band was observed, after 20 min of exposure.§Pα, PDGFRα; Pβ, PDGFRβ; V1, VEGFR1; V3, VEGFR3; WT, Wild Type.