Dynamics of calcineurin-regulated gene expression in S. cerevisiae

Gene expression was quantified in crz1 delete S. cerevisae cells over a time course following calcineurin activation by CaCl2. Cells were pretreated with buffer or the calcineurin inhibitor FK506 to allow the identification of calcineurin-regulated genes. A strain with a calcineurin-resistant mutation in the transcription factor Hcm1 was also examined. We show that calcineurin activation leads to downregulation of a many clusters of cell cycle-regulated genes, including those expressed at the G1/S and G2/M transitions. For each strain/condition seven samples were collected at 10-30 minute intervals through 90 minutes following the addition of CaCl2. Duplicate time course experiments were performed for each strain/condition.