Data files and variable descriptions (in readMe)

We present a newly developed design for a self-contained benthic chamber for conducting in situ ecosystem experiments in streams, with a focus on biogeochemical processes such as ecosystem metabolism and nutrient cycling. Our design is complementary to smaller, portable chamber designs, and is meant to answer questions at larger scales. These new chambers allow for a high level of experimental control in the field and can be used to generate spatially explicit data regarding ecosystem processes and to test mechanistic hypotheses. They are built to be deployed within the stream over periods of weeks to months and to withstand natural hydraulic forces of the benthic zone. First, we describe the materials and steps that are needed to construct these chambers in detail. Then, we report the methods and results of a multi-part, diagnostic field study meant to demonstrate the performance and utility of the design. We quantified solute dynamics using a conservative tracer injection, then we estimated ecosystem metabolism across the study site and performed nutrient additions. The field study revealed that both flow velocity and water depth impose limitations on these chambers, but with appropriate methodological forethought these limitations can be minimized. The capacity of our design to accommodate complex, three-dimensional habitats and macrofauna, along with the capability to generate spatially explicit data, are the main advances we present. These advances provide a novel method whereby motivated users can connect mechanistic hypothesis testing with natural ecological processes through ecosystem-level field experiments.