Absorption spectra of soybean measured in situ at two different temperatures and electron transport rate data

The dataset was collected using the leaf chamber spectrometer connected to an infrared greenhouse gas analyser (Li-6400) on soybean (<i>Glycine max</i> L. Merr.) cultivar (Williams 82) third trifoliate leaves on 3-week old plants (vegetative growth stages V3 and V4). Seeds were sourced from Iowa State University, USA. Plants were grown in pots in the controlled environment facility in the Faculty of Biological Sciences, Plant Growth Suite, University of Leeds. The growth conditions were 25C day/night temperatures, 50% relative humidity, with a 16 h photoperiod at an irradiance of 400–600 μmol photons /m²/s.<br>Electron transport rates were measured on a separate set of plants using a leaf chamber fluorometer (Li-6400-40) attached to the infrared greenhouse gas analyzer (Li-6400). Leaves were exposed to temperatures between 25°C and 35°C in the leaf chamber. Each temperature point was allowed to stabilize for a minimum of 7 minutes. The leaves experienced increasing temperatures (25, 27, 30, 33, and 35°C) and for comparable durations before reaching 35°C. Hence, the data presented for the ETR are different in duration of treatment to the 15-minute fixed duration treatment experiments.