Effect of peripherally-derived macrophages in adult microglia (mouse cells). Mus musculus

Infiltrating monocyte derived macrophages (MDMs) and resident microglia dominate CNS injury sites. We show that MDMs and microglia can directly communicate to modulate each other’s function. Also, the presence of MDMs in CNS injury suppresses microglia-mediated phagocytosis and inflammation. We suggest that macrophages infiltrating the injured CNS provide a mechanism to control acute and chronic microglia-mediated inflammation, which could otherwise drive damage in a variety of CNS conditions. To understand the global effects of macrophage communication to microglia, we transcriptionally profiled activated adult mouse microglia in the presence or absence of macrophages with and without an inflammatory stiumulus (LPS) Overall design: Bone marrow-derived macropages and adult microglia from C57BL/6 male mice were cultured seperately for 7 days. On the 7th day, cells were placed together in a bilmaniar culture sytem for 24h hours, with or without an inflammatory stimulus (LPS)