SRCAP mutation promotes cutatneous squamous cell carcinoma invasion [ChIP-Seq]

In this study, we identify a mutational hotspot affecting SRCAP in cancer and that SRCAP is recurrently mutated in keratinocyte carcinomas. When introduced to primary human epidermal keratinocytes, the SRCAP hotspot mutation dysregulates gene expression in the differentiated state far more than the progenitor state. Gene dysregulation occurs without a change in H2A.Z chromatin occupancy. Finall, the presence of the SRCAP mutation in a RAS-CDK4 driven model of cSCC increases invasion significantly. This is H2A.Z ChIP-seq data in primary human keratinocytes expressing the identified SRCAP mutation in progenitor and differentiated states.