Transcription profiling by array of mouse embryonic hearts of Numbl knockout and wild type

The ventricular wall of the heart is composed of trabeculated and compact layers, which are separated by yet unknown processes during embryonic development. Notch signaling plays important roles in cardiac development. Mutations in Notch signaling components are related to human congenital heart diseases Two homologues, Numb and Numblike (Numbl), are expressed in mammals, which also act as inhibitors of Notch signaling Here we investigate the role of Notch2 and Numb/Numblike during myocardial trabeculation and compaction. The aim of the experiment is to analyze whether Numb/Numblike are involved in inhibition of Notch activity in the developing heart.心脏心室壁由肌小梁层（trabeculated layer）与致密层（compact layer）构成，二者在胚胎发育过程中的分离机制目前仍未阐明。Notch信号通路（Notch signaling）在心脏发育中发挥关键调控作用。Notch信号通路组分的突变与人类先天性心脏病密切相关。哺乳动物体内存在两种同源蛋白——Numb与Numblike（Numbl），二者同样可作为Notch信号通路的抑制剂。本研究旨在探究Notch2以及Numb/Numblike在心肌肌小梁形成（myocardial trabeculation）与致密化（compaction）过程中的功能。本实验的核心目的为分析Numb/Numblike是否参与调控发育中心脏内的Notch活性抑制过程。