The Genome Resolved Open Watersheds database (GROWdb V2)

This repository contains data for version 2 of the Genome Resolved Open Watersheds database (GROWdb), along with data for the manuscript describing functional response of river sediment microbiomes to environmental stressors and key MAG metabolic functions. This includes data on both, the gene database and the MAG database. "Conserved adaptation to environmental factors by stream microbiomes in the hyporheic zone across North America" Tom L. Stach, Jörn Starke , Feriel Bouderka, Till L. V. Bornemann, André R. Soares, Michael J. Wilkins, Amy. E. Goldman, James C. Stegen, Mikayla A. Borton and Alexander J. Probst GROWdb Global MAG files GROWdbv2_MAG_dereplicated_MAGs.tar.gz- Fasta files of MAGs. GROWdbv2_MAG_dRep_DRAMv2_annotation.tsv- Functional annotation of GROWdb v2 MAGs using DRAMv2 (beta) GROWdbv2_MAG_dRep_gtdbtk_tax.summary.tsv- Taxonomic annotation of GROWdb v2 MAGs using GTDB-TK (v2.4.0) based on database r220. GROWdb Global Gene files GROWdbv2_Gene_DB_95AAI_no_singeltons.genes.faa.gz- FAA file of genes which make the gene DB. GROWdbv2_Gene_DB_95AAI_no_singeltons_annotation_FunTaxDB1.4.b6_cut_annotation.tsv- Functional annotation of GROWdb v2 Gene DB (95% AAI) using FunTaxDB (v1.4 | https://zenodo.org/records/7180192#.Y6Mjn-LMLtN) GROWdbv2_Gene_DB_95AAI_no_singeltons_annotation_DRAM_extract_KEGG_id.annotation.tsv- Functional annotation of GROWdb v2 Gene DB (95% AAI) using DRAMv2 (beta). Only KEGG hits are extracted.