Lactate-mediated histone lactylation promotes melanoma angiogenesis

The pathogenesis of cancer is rather complicated and includes multiple aspects, with metabolic reprogramming and angiogenesis as the leading hallmark characteristics. Recent reports have unveiled that the glycolytic metabolite lactate could modify histone lactylation level to epigenetically regulate gene expressions and biological processes in cancer, while the role of lactate-mediated histone lactylation in tumor angiogenesis remains elusive. By taking advantage of melanoma as the model, we demonstrate that lactate-mediated histone lactylation promotes tumor angiogenesis via IL-33/ST2 axis. Overall design: In order to identify the key key genes regulating angiogenesis through histone lactylation,Transwell chamber is used to co-culture HUVECs with human melanoma cell line A2058 for 72 hours to establish TA-HUVECs,Then TA-HUVEC treated with NALA (10 mM) and/or A-485 (10 µM) for 48h.