Modular organization of the motor space in the mouse superior colliculus

In order to characterize the genetic diversity within the Stratum Griseum Intermediale (SGI), we screened gene expression by extracting RNA from sensory and motor layers of the Superior Colliculus (SC) at post-natal day (P)7. Sensory and motor domains were manually dissected from P7 mice in order to obtain four samples from each mouse: sensory anterior SC, sensory posterior SC, motor anterior SC and motor posterior SC. RNA was extracted from these samples RNeasy Mini kit (Qiagen, Venlo, Netherlands) and cDNA libraries were generated from total RNA using a TruSeq® Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA) and sequenced on an Illumina HiSeq 4000 machine (single read, 50bp read length).