Characterization and quantification of AAV capsid loading states by multi-wavelength AUC with UltraScan - supplementary material

SF 1: Spectral deconvolution fits for the MW data of AAV8 at scan 35SF 2: Screen shot of the spectral decomposition residual plot in UltraScan demonstrating the fit and residuals at individual radius positions for all wavelengths measured for AAV8.SF 3: Extinction spectrum for AAV5 capsid protein (blue) and DNA (red) between 230-310 nm. Absorbance spectra are normalized for to have the same area under each curve.SF 4: Sample quantity requirements – SV vs. ABDE. Required sample amounts are proportional to the orange shaded regions in an SV experiment (top) and an ABDE experiment (bottom). Since ADE experiments collect the entire signal only in the peak area, sample amounts are significantly reduced. In both experiments, the dynamic range of the detector must be observed to insure a linear response. In the case shown here, the amount of sample required for an ABDE experiment is 23.7 fold lower than in the SV experiment when integrating the orange area for both plots. This ratio increases if fewer peaks are observed, or decreases when additional peaks are observed.