Alu element-containing RNAs maintain nucleolar structure and function. Alu element-containing RNAs maintain nucleolar structure and function

We have isolated, analyzed and compared the RNA content of various cell compartments including total RNA, nucleolplasmic RNA and nucleolar RNA. In addition, cells have been subjected to various treatment to specifically inhibit each of the RNA polymerases I, II and III, as well as protein synthesis. Cells were also treated with antisense oligos to induce the degradation of specific RNA transcripts. The whole RNA content of the cells after these specific treatments was isolated and sequenced.