The tumor suppressive role of ANCO1 in early-stage triple negative breast cancer [ChIP-seq]

To examine the impact of ANCO1 reduction on H3K27Ac modification and its association with gene expression changes, we performed ChIP-seq experiments in shANCO1 and control MCF10A and MCFDCIS cells. We report ANCO1 knockdown causes global changes to H3K27Ac pattern and enhances accessibility of breast cancer enhancers. Altered H3K27Ac signal is associated with gene expression changes. ANCO1 knockdown MCF10A and MCFDCIS cells were generated with lentiviral infection. ChIP-seq experiments were performed in shANCO1 and shCTRL cell lines to examine H3K27Ac engagement in the genome. Experiments were done in triplicates. Immunoprecipitated samples were normalized to respective input controls.