Assembly of pitcher plant microbiomes 2016

Aim
Ecological theory suggests that dispersal limitation and selection by climatic factors influence bacterial community assembly at a continental scale, yet the conditions governing the relative importance of each process remains unclear. The carnivorous pitcher plant Sarracenia purpurea provides a model aquatic microecosystem to assess bacterial communities across the host plant's North–South range in North America. This study determined the relative influences of dispersal limitation and environmental selection on the assembly of bacterial communities inhabiting S. purpurea pitchers at the continental scale.
Location
Eastern United States and Canada.
Time period
2016
Major taxa studied
Bacteria inhabiting S. purpurea pitchers.
Methods
Pitcher morphology, fluid, inquilines and prey were measured, and pitcher fluid underwent DNA sequencing for bacterial community analysis. Null modeling of β-diversity provided estimates for the contributions of selection and dispersal limitation to com..., , , # Assembly of pitcher plant microbiomes

## 16S DNA sequencing data

The raw sequence reads are available on the SRA database under PRJNA641293.

## Description of the data and file structure

The environmental data needed to reproduce the analyses in the manuscript are saved as \"environmental-variables.txt\".

Sample_name: the name of the sample, corresponding with the DNA sequencing files
site_id: the identification code for the site from which the sample was collected
rosette_diameter_1: a measurement of the pitcher rosette (mm)
rosette_diameter_2: a measurement of the pitcher rosette (mm)
pitcher_length: a measurement of the length of the pitcher (mm)
pitcher_width: a measurement of the width of the pitcher (mm)
keel_width: a measurement of the pitcher's keel (mm)
mouth_diameter: a measurement of the diameter of the pitcher's mouth (mm)
chlorophyll: a measurement of the pitcher chlorophyll (SPAD units, representing the difference in optical density at two wavelengths)
volume: a mea...