RobynvanZijl_IRMS_Plant

This dataset contains carbon and nitrogen stable isotope ratios (δ¹³C, δ¹⁵N) and elemental percentages (%C, %N) for plant samples collected across 4 sites in the Berg River Estuary in June 2025. Analyses were conducted at the BIOGRIP Water and Soil Node, Stellenbosch University, using a Vario Isotope Select elemental analyser (Elementar, Germany) coupled to an Isoprime precision isotope ratio mass spectrometer (IRMS, Elementar, UK) with LyticOS© software (v5.0.4.118). Samples were weighed into tin capsules (approximately 2–3 mg) and analysed following standard EA-IRMS protocols, including combustion, reduction, drying, and separation via a Temperature Programmed Desorption (TPD) column. δ¹³C values are expressed relative to Vienna Pee Dee Belemnite (VPDB), and δ¹⁵N values relative to atmospheric nitrogen (Air). Quality control and drift correction were achieved using reference standards (USGS40, Coldwater Fish Gelrite) and laboratory standards (wheat flour, sorghum flour), analysed periodically throughout runs.