RNA-seq analysis for zebrafish tumors driven by overexpression of human IRF4

RNA-seq analysis was performed using zebrafish primary tumors and control thymus to analyze gene expression changes after IRF4 overexpression. Overall design: Human IRF4 gene was overexpressed under the zebrafish lck promoter. Tumor cells were harvested from IRF4-transgenic zebrafish (lck-IRF4) with or without heterozygous p53 mutation. Normal thymus cells were also harvested from control zebrafish (lck-mCherry).