tRF/tiRNA expression profile of RPE-choroid-sclera complex in the laser-induced choroidal neovascularization mouse model

To investigate the tRF/tiRNA expression pattern in the development of choroidal neovascularization, we built a laser-induced choroidal neovascularization mouse model and performed the tRF/tiRNA sequencing on RPE-choroid-sclera complexes at different time points after model establishment, while the tissues from mice without treatment were used as control group. Overall design: Three RPE-choroid-sclera complexes were obtained from the eyes of C57BL/6 mice at day 3 and day 7 after the laser-iduced choroidal neovascularization model establishment. Three complexes from mice without treatment were used as control samples. All nine samples were used for tRF/tiRNA sequencing and data analysis.