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LC-MS/MS after Immunoprecipitation with Mbd5 in zebrafish at 4dpf

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NIAID Data Ecosystem2026-05-02 收录
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To address how Mbd5 binding to m5C RNAs mediates transcriptional activation of its target genes. Using the Tg[zhsp70l:FLAGmbd5iso2-E2AGFP] zebrafish, we performed heat shock at multiple developmental stages and carried out co-immunoprecipitation (co-IP) at 4 dpf using the FLAG antibody, followed by mass spectrometry

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2024-06-16
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