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In vitro development of enucleated oocytes injected with freeze-dried and fresh, control granulosa cells.

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The culture were maintained for 7–8 day in medium SOF plus amino-acids and BSA, with FCS added a day 4. reconstructed embryos were checked every 24 hours for development. Test χ2 aP = 0.88; bP = 0.17; cP = 0.56

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2013-02-21
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