Evolved Cas9 variants with broad PAM compatibility and high DNA specificity
收藏NIAID Data Ecosystem2026-05-25 收录
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Evolved xCas9 with broader PAM compatibility and greater DNA specificity.
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创建时间：
2018-04-06
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CAS9MODIFICADA MOLECULE ALONE， CAS9MODIFICADA， METHOD FOR PRODUCING A PROTEIN OR RIBONUCLEOPROTE??NA COMPLEXES OR COMPLEXES OF PROTEIN and RIBONUCLEOPROTE??NA MIXED OR LIP??DEO， FUSION PROTEIN， NUCLEOTIDE SEQUENCE， NUCLEIC ACID， VECTOR， CELL， ANIMAL OR PLANT， PHARMACEUTICAL COMPOSITION， IN VITRO USE OF A RECOMBINANT CAS9MODIFICADA， KIT OF PARTS， and IN VITRO METHOD FOR ALTERING THE GENOME OF A CELL
To address the limitations deriving from the unspecific genomic cleavages of the Streptococcus pyogenes Cas9 (SpCas9) and to identify variants with higher cleavage fidelity， the present invention des
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Straightforward Delivery of Linearized Double-stranded DNA Encoding sgRNA and Donor DNA for the Generation of Single Nucleotide Variants Based on the CRISPR/Cas9 System
CRISPR/Cas9 for genome editing requires delivery of a guide RNA sequence and donor DNA for targeted homologous recombination. Typically, single-stranded oligodeoxynucleotide, serving as the donor temp
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Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing
Biochemical, biophysical and cell-based assays on S. pyogenes Cas9 (SpyCas9) variants with wide-ranging genome editing efficiencies that differ in PAM binding specificity to uncover the molecular dete
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Additional file 3: of NmeCas9 is an intrinsically high-fidelity genome-editing platform
Table S2. Summary of potential off-target sites detected by GUIDE-seq analysis using the Bioconductor package GUIDE-seq. The table includes sites for both NmeCas9 (with N4GN3 PAM sequences) and SpyCas
Figshare2024-02-08 更新70
Developing small Cas9 hybrids using molecular modeling
The RNA-guided CRISPR-Cas9 from Streptococcus pyogenes is the best characterized enzyme for gene editing. Its large size, however, precludes it from being packaged together with its single guide (sg)R
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