Supplementary Material for: Blue Dye-Assisted Intraoperative Optical Coherence Tomography for Macular Surgery

Introduction: The purpose of this study was to evaluate whether vital blue dyes could enhance the contrast of intraoperative OCT during macular surgery.
Methods: Consecutive patients undergoing elective pars plana vitrectomy for vitreomacular interface disorders were enrolled. Intraoperative OCT was performed with the the Artevo 800 microscope (Carl Zeiss Meditec AG, Jena, Germany) before and after injection of 0.2 mL of Trypan Blue and Brilliant Blue G ophthalmic solution. The OCT contrast ratio was measured with ImageJ, while the overall scan quality was subjectively classified using a 4-point scale.
Results: Ten eyes of 10 patients were enrolled in the study. The OCT contrast ratio was 9.39 ± 5.35 without blue dye, and significantly improved to 14.31 ± 10.50 after blue dye injection (P = 0.027). The percentage of patients with a grade 4 scan quality also significantly improved (from 40% without blue dye to 90% with blue dye injection; P = 0.012).
Conclusion: The use of blue dyes during intraoperative OCT is an effective strategy for improving contrast and scan quality without affecting the surgical time and workflow.