Bone marrow HSPCs differentiate into myeloid cells in depression

It has been known that the numbers of neutrophils and Ly6Chi monocytes are increased in the blood of patients with depression. To better understand how HSPCs sense and adapt disease progression in depression, we used single-cell RNA sequencing (scRNA-seq) to characterize HSPCs in bone marrow from mice models of depression induced by chronic restraint stress (CRS) and chronic unpredictable mild stress (CUMS).We found that bone marrow hematopoiesis was substantially rewired toward myeloid lineages in depression, accompanied by an increase of myeloid score in bone marrow HSPCs, suggesting myeloid biased cell production in bone marrow of depressed mice. We performed single-cell RNA sequencing on flow cytometry-sorted LK cells from the bone marrow of mice exposed to CRS and CUMS.