The majority of messenger RNA precursor (pre-mRNA) undergoes 3' end cleavage and polyadenylation (CPA), termed as to 3’ end processing. This processing is specified by a polyadenylation site (PAS) and

NIAID Data Ecosystem10

Evolutionary dynamics of polyadenylation signals and their recognition strategies in protist. Evolutionary dynamics of polyadenylation signals and their recognition strategies in protist

RNA加工机制

序列进化

Cleavage and polyadenylation are the final steps of eukaryotic mRNA 3' end formation. The most critical element in humans and other model organisms is the poly(A) signal, an AAUAAA hexamer. We recentl

NIAID Data Ecosystem00

Sequences that influence mRNA trans splicing and polyadenylation in trypanosoma brucei.. Sequences that influence mRNA trans splicing and polyadenylation in trypanosoma brucei.

RNA加工机制

布氏锥虫基因表达

Trypanosoma brucei library consisting of a pool of reporters with the 14Ts polypyrimidine tract replaced with 11 random nucleotides. The library is treated with 0.2ug/ml of puromycin, then survivors a

NIAID Data Ecosystem20

Identification of COUP-TFII interacting proteins in MCF-7 cells.

蛋白质相互作用

细胞分子生物学

WCE from pCOUP-TFII-FLAG-transfected MCF-7 cells (Fig. 1) was incubated with anti-FLAG affinity gel, eluted with 0.1 M glycine, pH 3.5 for 5 min. at 95°C, and subjected to MudPIT peptide identificatio

NIAID Data Ecosystem10

Global identification of target recognition and cleavage by the Microprocessor in human ESCs.

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