Expression data from CHO-K1 cells in putrescine-deprived conditions

CHO cells grown in serum-free medium have an absolute requirement for putrescine supplementation due to their deficiency in activity of the enzyme arginase. Consequently, principally in this system, polyamines play a central but poorly-understood role in cell proliferation Cell growth arrest, mainly in the S- and G2/M phases was observed. We used differential gene expression data to investigate the impact of polyamine deprivation on the transcriptome of CHO cells CHO-K1 cells were grown in media deprived of putrescine (and any other polyamine and ornithine) for three days. A control culture, was grown in paralell in the same media but containing putrescine. Cell growth, viability and cell cycle were analysed during the three days. At day 3, samples for RNA extraction were collected from the biological triplicates of both conditions and hybridased in an Affymetrix microarray chip.