Levels of Candida Sap1/Sap2/Sap3 in the culture supernatant of the selected clinical <i>C</i>. <i>albicans</i> strains.

The selected C. albicans clinical strains and of the reference SC5314 strain were cultivated in YPD medium overnight at 30°C. Culture supernatant derived from 1x106 cells of each C. albicans strain was separated by SDS-PAGE and transferred to a membrane and developed with a polyclonal rabbit anti Sap1/Sap2/Sap3 serum, followed by a HPR swine anti-rabbit serum as a secondary antibody. The 42 kDa Sap2 band was detected in culture supernatant derived from one low, two medium and one high Gpm1/Pra1 expressing isolate. The figure shows a representative experiment out of three performed. (DOC)