Pelagic eukaryotic communities across Fram Strait

Water samples were collected in Fram Strait during the Polarstern cruise PS85 (June 6th 2014
until July 3rd 2014) from eastern Greenland shelf to west coast of Spitsbergen. For assessing eukaryotic
community composition 2 L of water were filtered using vacuum filtration (at 200 mbar) through
successive membrane filters of 10 m , 3 m and 0.4 m. The extracted genomic eukaryotic DNA was pulled
together from the different fractions and the hypervariable V4 (528iF - 964iR) region of the 18S rRNA
was amplified and sequenced using Illumina MiSeq platform in a 2 300 bp paired-end run.

在极地星号号航次PS85（2014年6月6日至7月3日）期间，于弗拉姆海峡收集了水样，样本范围从东格陵兰架至斯匹次卑尔根岛西海岸。为评估真核生物群落组成，使用真空过滤（200毫巴）方法，通过10微米、3微米和0.4微米的依次膜滤器对2升水样进行过滤。将提取的基因组真核生物DNA从不同组分中收集并集中，利用Illumina MiSeq平台进行扩增和测序，该平台采用双端测序模式，测序长度为300碱基对，针对18S核糖体RNA基因的hyper-variable V4区域（528iF-964iR）进行测序。