ATAC-seq of bone marrow neutrophil treated PGE2 or Vehicle

Purpose: Investigate the gene accessibility change of neutrophils from PGE2 stimulation and identify the posibility for PGE2 could induce lung neutrophil like characteristics. Methods: Isolated bone marrow neutrophils with Percoll gradient are treated with 10 μM of PGE2 for 24 hours. Conclusion: Chromatin accessibility for the genes that were upregulated in lung neutrophils and PGE2 treated neutrophils were increased, so PGE2 could induce lung neutrophil like characteristics with change chromatin accessibility. ATAC-seq analysis of neutrophils isolated bone marrow were treated with 10 μM PGE2 or vehicle. 3 biological replicates.