Original data

Our study elucidates a partial mechanism underlying inflammation in busulfan‑induced oligozoospermic mice, implicating the S100A9/TLR4/NF‑κB pathway in testicular inflammation. Administration of Tyr significantly reduces S100A9 and TLR4 expression in the testis, thereby ameliorating inflammation. Additionally, Tyr improves sex hormone balance, oxidative stress levels, and inflammatory status, ultimately Oenhancing sperm concentration and restoring testicular tissue architecture in oligozoospermia. Future studies will focus on cellular‑level investigations to elucidate the specific effects of Tyr on different testicular cells.本研究阐明了白消安（busulfan）诱导的少精子症小鼠炎症反应的部分潜在机制，提示S100A9/TLR4/NF-κB通路（S100A9/TLR4/NF-κB pathway）参与了睾丸炎症的病理进程。给予酪氨酸（Tyr）可显著下调小鼠睾丸组织中S100A9与TLR4的表达水平，从而减轻炎症反应。此外，酪氨酸可改善性激素平衡状态、调控氧化应激水平并缓解炎症，最终提升少精子症模型小鼠的精子浓度，同时修复受损的睾丸组织结构。未来本研究将聚焦细胞层面的实验，以阐明酪氨酸对不同睾丸细胞的具体作用效应。