Gene expression profiles of immature versus mature bone marrow-derived basophils (BMBAs) elicited by either IL-3 or TSLP.

In this study, we explored gene expression profiles of mature and immature basophils from either IL-3-elicited or TSLP-elicited BMBAs. We revealed that TSLP-elicited mature basophils displayed gene expression profile similar to that of IL-3-elicited mature rather than immature basophils. Overall design: We cultured bone marrow cells in thepresence of IL-3 or TSLP for 7 days. We then isolated immature and mature basophils from either IL-3-cultured or TSLP-cultured bone marrow cells.